Solution Information | help | |
Enzyme: | Inositol-trisphosphate 3-kinase A | |
inhibitor: | BDBM82312 | |
substrate: | n/a | |
Solution Type: | Aqueous | |
pH at Preparation: | 8.0 | |
Temp. Prep.: | 37 | |
Comments: | n/a | |
pH Buffer 1: | Tris-Cl | |
Concentration: | 50 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 2: | KCI | |
Concentration: | 100 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 3: | DTT | |
Concentration: | 1 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 4: | 2,3-diphosphoglycerate | |
Concentration: | 1 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 5: | D-I(1,4,5)P3 | |
Concentration: | 20 uM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 6: | ATP | |
Concentration: | 50 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 7: | EGTA | |
Concentration: | 2 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 8: | [3H](1,4,5)P3 | |
Concentration: | 20,000 dpm | |
ΔHion: | n/a | |
Comments: | n/a | |
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